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goat serum  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology goat serum
    Goat Serum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+serum/Triton+X-100/pmc12933830-129-6-23
    Average 96 stars, based on 11727 article reviews
    goat serum - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Cognitive neurobehavior and synaptic plasticity after vagus nerve stimulation in female rats: Interaction between estrous cycle and neurostimulation
    Article Snippet: .. Tissue was blocked (PBS + 2% goat serum, 0.2% Triton X-100) for 1 h at room temperature and incubated in rabbit anti-BDNF (1:250, sc-20981, Santa Cruz) overnight at 4 °C. .. The next day the tissue was incubated in mouse anti-NeuN (1:2500, MAB377, Sigma) for 2 h at room temperature.

    Article Title: Lysosome-targeting live attenuated influenza vaccines elicit robust and broad immunity in mice
    Article Snippet: .. After another 6 h of culture, the cells were washed with PBS, fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature, washed with PBS, permeabilized with PBS containing 0.1% Triton X-100 for 5 min, blocked with PBS containing with 0.1% Triton X-100 and 10% goat serum for 1 h at room temperature, and incubated with anti-Flag antibody (MBL, Cat# PM020; 1:1000 dilution) and anti-HSC70 antibody (Santa Cruz Biotechnology, Cat# sc-7298; 1:300 dilution) or anti-LAMP2A antibody (Santa Cruz Biotechnology, Cat# sc-18822; 1:300 dilution) diluted in PBS containing 0.1% Triton X-100 and 1% goat serum overnight at 4 °C, followed by incubation with Alexa Fluor 488 goat anti-rabbit IgG (Life Technologies, Cat# A11034; 1:1000 dilution) and Alexa Fluor 555 goat anti-mouse IgG (Life Technologies, Cat# A32727; 1:1000 dilution) for 1 h at room temperature. .. Cell nuclei were counterstained with DAPI (Invitrogen, Cat# 2086723) after the secondary antibody staining.

    Article Title: The CD74-MIF axis contributes to acute uveitic injury by upregulating proinflammatory cytokine secretion in murine cytomegalovirus intracameral infection mouse models.
    Article Snippet: Apoptotic cells were visualized using a fluorescence microscope (Carl Zeiss, Japan). .. For immunofluorescence, sections were deparaffinized, rehydrated, antigen retrieved, blocked with goat serum, permeated and incubated with primary antibodies (mouse anti-CD45, Proteintech Biotechnologies; anti-CD74, Santa Cruz Biotech). ..

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. After permeabilization and blocking with 10% goat serum containing 0.2% Triton X-100, the sections were incubated with primary antibodies against ADGRG1 (1:50; sc-390192, Santa Cruz Biotechnology), TOM20(11802-1-AP, Proteintech), 4-HNE(68538-1-Ig, Proteintech), 4-HNE(HY-P81208, MCE), Ferritin (Rockland 200-401-090-0100), LAMP1(65051-1-Ig, Proteintech), PAX1(sc-514352, Santa Cruz Biotechnology), FOXF1(PA5-83039, Thermo Fisher). ..

    Article Title: Mammalian senescent nurse cell provides Trichinella spp. muscle larvae with cell growth inhibitory capacity.
    Article Snippet: Muscle larva of the mammalian parasitic nematode Trichinella spp. resides in a single cell called a nurse cell (NC).. Despite originating from muscle cells the NC is of the non-muscular type.. Its only role is to sustain muscle larva throughout the host’s life.

    Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.
    Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 ◦C with the following primary antibodies: anti-NANOG (bs0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1- AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

    Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells
    Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 °C with the following primary antibodies: anti-NANOG (bs-0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1-AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

    other:

    Article Title: Exogenous visfatin suppresses pituitary gonadotrophins and stimulates testosterone secretion in a male mouse.
    Article Snippet: Correspondence Vikas Kumar Roy, Department of Zoology, Mizoram University, Aizawl, Mizoram 796004, India.. Email: vikasroy4araria@yahoo.co.in Abstract Visfatin expression has been shown in the testis and pituitary.. However, the role of visfatin in the pituitary and testis axis is fragmentary.

    Immunofluorescence:

    Article Title: The CD74-MIF axis contributes to acute uveitic injury by upregulating proinflammatory cytokine secretion in murine cytomegalovirus intracameral infection mouse models.
    Article Snippet: Apoptotic cells were visualized using a fluorescence microscope (Carl Zeiss, Japan). .. For immunofluorescence, sections were deparaffinized, rehydrated, antigen retrieved, blocked with goat serum, permeated and incubated with primary antibodies (mouse anti-CD45, Proteintech Biotechnologies; anti-CD74, Santa Cruz Biotech). ..

    Blocking Assay:

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. After permeabilization and blocking with 10% goat serum containing 0.2% Triton X-100, the sections were incubated with primary antibodies against ADGRG1 (1:50; sc-390192, Santa Cruz Biotechnology), TOM20(11802-1-AP, Proteintech), 4-HNE(68538-1-Ig, Proteintech), 4-HNE(HY-P81208, MCE), Ferritin (Rockland 200-401-090-0100), LAMP1(65051-1-Ig, Proteintech), PAX1(sc-514352, Santa Cruz Biotechnology), FOXF1(PA5-83039, Thermo Fisher). ..

    Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.
    Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 ◦C with the following primary antibodies: anti-NANOG (bs0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1- AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

    Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells
    Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 °C with the following primary antibodies: anti-NANOG (bs-0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1-AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

    Saline:

    Article Title: Mammalian senescent nurse cell provides Trichinella spp. muscle larvae with cell growth inhibitory capacity.
    Article Snippet: Muscle larva of the mammalian parasitic nematode Trichinella spp. resides in a single cell called a nurse cell (NC).. Despite originating from muscle cells the NC is of the non-muscular type.. Its only role is to sustain muscle larva throughout the host’s life.



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